解剖学和形态学
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听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
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神经系统科学
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产科医学和妇科医学
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生理学
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泌尿学和肾脏学
病毒学
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健康政策和服务
心理学,临床
abstract::Insulin receptors from rat hepatoma cells (Fao) and human placenta were partially purified by detergent solubilization and lectin purification. The insulin receptor preparations were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis under reducing or nonreducing conditions. The proteins were...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.10.923
更新日期:1984-10-01 00:00:00
abstract::Enhancement of arachidonic acid metabolism results in increased insulin secretion. To determine which pathways of arachidonic acid metabolism were involved in this stimulation, we studied the effects of various inhibitors of arachidonate metabolism on arginine-induced insulin and glucagon secretion in the isolated, pe...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.10.929
更新日期:1984-10-01 00:00:00
abstract::The effect of glyburide on glucose metabolism was examined in 10 non-insulin-dependent diabetic subjects (NIDDM) and 7 young, control subjects. After 3 mo of glyburide treatment in NIDDM, fasting plasma glucose declined from 198 to 141 mg/dl (P less than 0.01) without change in fasting insulin levels. Basal hepatic gl...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.9.838
更新日期:1984-09-01 00:00:00
abstract::Platelet aggregation and related plasma factors have been studied in experimentally diabetic dogs, experimentally galactosemic dogs, and in normal dogs. Platelet aggregation, when induced in vitro by ADP (up to 22 microM) or collagen (up to 4 micrograms/ml), was not significantly different from normal in the diabetic ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.9.846
更新日期:1984-09-01 00:00:00
abstract::The interaction between insulin and its receptor was investigated using both monoclonal and polyclonal anti-insulin antibodies. After covalent cross-linking of 125I-insulin to the insulin receptor on cultured human lymphocytes (IM-9 cells) using disuccinimidyl suberate, we inquired whether the insulin-receptor complex...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.8.778
更新日期:1984-08-01 00:00:00
abstract::Platelets from diabetic subjects with circulating immune complexes (CIC) synthesized greater amounts of thromboxane than did platelets from CIC-negative patients or controls. In view of the known action of CIC on platelet function, a relationship between these two factors may be suggested in the initiation and progres...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.8.728
更新日期:1984-08-01 00:00:00
abstract::We studied certain metabolic requirements for insulin-induced increases in phospholipids, and the relationship of phospholipid changes to the insulin-induced activation of pyruvate dehydrogenase, in rat adipocytes and fat pads in vitro. Increases in the contents of phosphatidylinositol and phosphatidylserine mass were...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.7.648
更新日期:1984-07-01 00:00:00
abstract::The relative effects of time versus ambient glucose concentration on insulin-stimulated glucose uptake was estimated by performing 5-h insulin clamp studies in patients with NIDDM. Each experimental subject was studied three times, at steady-state plasma insulin levels approximately 2000 microU/ml, but at different st...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.7.643
更新日期:1984-07-01 00:00:00
abstract::The presence of the enzyme aldose reductase is increasingly being linked to diabetic complications. The distribution of this enzyme in human cornea, lens, retina, and optic nerve has been studied using specific antibodies against purified human placental aldose reductase raised in both rabbit and goat. The antisera fr...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.6.562
更新日期:1984-06-01 00:00:00
abstract::The effect of nonenzymatic glycosylation on the biologic function of human antithrombin III was evaluated using a chromogenic thrombin substrate assay in the presence of catalytic amounts of heparin. Experimental conditions that increased the rate of nonenzymatic protein glycosylation were associated with decreases in...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.6.532
更新日期:1984-06-01 00:00:00
abstract::Total lymphoid irradiation (TLI) at doses of 2200 rads or greater prevented diabetes in susceptible BB/W rats. Two of 29 (7%) treated rats became diabetic compared with 23 of 39 (59%) controls (P less than 0.001). TLI did not, however, prevent insulitis or thyroiditis in nondiabetic rats, nor did it restore the depres...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.6.543
更新日期:1984-06-01 00:00:00
abstract::A chronic diabetic state was produced in Macaca fuscatus , and these diabetic monkeys were kept without insulin treatment for up to 25 mo. The metabolic derangements were characterized by hyperglycemia, insulinopenia, hyperglucagonemia, ketonemia, and hyperlipidemia. Significant thickening of the capillary basement me...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.5.415
更新日期:1984-05-01 00:00:00
abstract::Blood T-cells from 28 patients with type I (insulin-dependent) diabetes (IDDM) of variable duration were examined for the Tac antigen by immunofluorescence, and for proliferation in the presence of interleukin 2 (IL 2). The mean percentage of Tac+ cells in patients whose IDDM was of less than 2-yr duration was 6.2% co...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.4.319
更新日期:1984-04-01 00:00:00
abstract::The relationship between the levels of circulating thyroid hormones and the action of insulin on adipose tissue was investigated in 6 hypothyroid patients and 6 hyperthyroid patients, all untreated, and 8 healthy control subjects. All were matched for age, body weight, and fat cell size. Gluteal s.c. adipose tissue wa...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.4.369
更新日期:1984-04-01 00:00:00
abstract::The uptake of 2-deoxy-D-[1-14C]-glucose, in the presence and absence of insulin, was measured in adipocytes from normal and streptozotocin-diabetic animals, over a wide temperature range (25-45 degrees C). Optimum temperatures for uptake in the presence of maximally stimulating insulin concentrations occurred near phy...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.3.258
更新日期:1984-03-01 00:00:00
abstract::Insulin requirements for meals were measured in eight insulin-dependent diabetic patients, using a closed-loop insulin infusion system. Patients required more insulin for breakfast than for an isocaloric lunch (35.7 +/- 5.5 mU/kcal/3 h versus 26.9 +/- 5.1 mU/kcal/3 h, P less than 0.02) or an isocaloric supper (35.7 +/...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.3.266
更新日期:1984-03-01 00:00:00
abstract::The pretreatment of isolated islets of Langerhans with concanavalin A (Con A) completely blocks alloxan from suppressing the insulin release response to glucose. The lectin itself inhibits insulin secretion. This effect is dose dependent and reversible. Con A, however, has no protective action against the inhibition o...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.2.164
更新日期:1984-02-01 00:00:00
abstract::Fourteen rats of the spontaneously diabetic BB line were bled from the retroorbital sinus approximately every 10 days. Sera taken from an early age up to 20 days after the onset of overt diabetes were assayed for complement-fixing antibodies against antigens of the surface of islet cells (CFA). Dispersed islet cells f...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.1.93
更新日期:1984-01-01 00:00:00
abstract::Diabetes-induced alterations in heme and hemoproteins, as well as its relationship to drug-mediated induction of ALA Synthase (ALA-S), were examined in female Sprague-Dawley rats. Animals were rendered diabetic by a single i.v. injection of streptozotocin (STZ, 65 mg/kg) and measurements were made at various times aft...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.1.37
更新日期:1984-01-01 00:00:00
abstract::Precise timing of the teratogenic period in diabetic pregnancy is of clinical importance since correction of the glucose intolerance during this period may protect the offspring from malformations. An experimental approach to elucidate this problem with regard to skeletal development was made in groups of pregnant str...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.12.1141
更新日期:1983-12-01 00:00:00
abstract::Sera from rats inoculated with in vitro glucosylated rat skin acid-soluble collagen were evaluated for specific antibody response using the enzyme-linked immunosorbent assay. The data indicate that the altered protein is capable of eliciting the production of antibodies, but unmodified collagen is not. The specificity...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.12.1182
更新日期:1983-12-01 00:00:00
abstract::Glucokinase from rat liver or transplantable, radiation-induced insulinomas was partially purified by ion exchange chromatography using DEAE-Cibacron Blue F3GA agarose. Phosphorylation of alpha,beta-D-mannose by glucokinase occurred with cooperative rate dependence on mannose concentration (nH: 1.50). Half-maximal pho...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.12.1146
更新日期:1983-12-01 00:00:00
abstract::Insulin receptors on viable rat adipocytes were affinity-labeled using a biologically active and photosensitive analogue of insulin, 125I-B2(2-nitro, 4 azidophenylacetyl)-des-PheB1-insulin (125I-NAPA-DP-insulin). The radiolabeled proteins were identified by SDS polyacrylamide gel electrophoresis and autoradiography. B...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.11.1001
更新日期:1983-11-01 00:00:00
abstract::Two major metabolic perturbations, increased polyol (sorbitol) pathway activity and reduced tissue myo-inositol content, are induced in peripheral nerve by hyperglycemia. Although they are commonly invoked as alternative biochemical pathogenetic mechanisms for diabetic neuropathy, their possible interrelationship has ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.11.988
更新日期:1983-11-01 00:00:00
abstract::Earlier investigations have revealed that polyethylene glycol-B1-insulin (PEG insulin) causes lower utilization of glucose in fat cells than does the unaltered hormone, even though the blood glucose-lowering activity of both preparations in intact animals is identical. The present study was aimed to establish whether ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.10.953
更新日期:1983-10-01 00:00:00
abstract::To assess the relationship between beta-cell function and the level and duration of hyperglycemia during generalized beta-cell impairment, we studied the effects of acute and prolonged infusion of somatostatin in seven normal men. Twenty minutes after beginning an acute infusion of somatostatin (200 microgram/h) plus ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.10.943
更新日期:1983-10-01 00:00:00
abstract::Ciglitazone, 5-[4-(1-methylcyclohexylmethoxy) benzyl]-thiazolidine-2,4-dione, is a new hypoglycemic agent orally active in the obese-hyperglycemic animal models. In C57BL/6J-ob/ob mice, treatment with 100 mg/kg ciglitazone for 2 days elicited a drastic fall in blood glucose. It also decreased plasma insulin, triglycer...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.9.830
更新日期:1983-09-01 00:00:00
abstract::Infection of autoimmune New Zealand mice with the D variant of encephalomyocarditis (EMC) virus results in beta-cell damage and clinical diabetes. The induction of diabetes in parental NZB and NZW strains was independent of sex. However, the susceptibility to virus-induced diabetes in their F1 offspring was sex depend...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.8.755
更新日期:1983-08-01 00:00:00
abstract::Livers from 9-10-wk-old genetically obese and hyperinsulinemic (fa/fa) rats contained more DNA, proteins, and lipids than livers of age-matched controls. Actually, the increase in liver mass of fa/fa rats was mainly due to an increased cell number. On perfusion with insulin (1.75-16 nM), livers of obese fa/fa rats rem...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.7.605
更新日期:1983-07-01 00:00:00
abstract::The effect of maternal diabetes on teratogenesis was examined utilizing female Chinese hamsters with typical diabetic symptoms from our closed colony (CHA) and F13-F19 of the brother-sister mating generations of a diabetic strain (CHAD). Both groups were mated with nondiabetic males from CHA. Embryologic studies on da...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.7.637
更新日期:1983-07-01 00:00:00
abstract::In man a small dose of somatostatin (50 micrograms/h) suppressed moderately basal insulin (5 microU/ml) and glucagon (40 pg/ml) levels. This resulted in a short-lasting hypoglycemia, which was then followed by marginal hyperglycemia throughout the experiment. The addition of a minimal dose of glucagon (0.50 ng/kg/min)...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.7.633
更新日期:1983-07-01 00:00:00
abstract::Rats with chronic streptozotocin (SZ) diabetes develop dilatation of the alimentary tract, loss of fecal consistency, and autonomic neuropathy involving unmyelinated axons of the extrinsic innervation of the small bowel. Diabetic autonomic neuropathy involving the ileal mesenteric nerves is characterized by modest to ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.6.532
更新日期:1983-06-01 00:00:00
abstract::To evaluate possible advantages of human insulin of recombinant DNA origin (HI) in the treatment of diabetic patients, we compared cellular and humoral immunoreactivities of HI and porcine insulin (PI). Anti-insulin IgE bound equal amounts of 125I-HI and 125I-PI. There was no difference between HI- and PI-stimulated l...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.6.516
更新日期:1983-06-01 00:00:00
abstract::Alterations in renal function and structure are found even at the onset of diabetes mellitus. Studies performed over the last decade now allow definition of a series of stages in the development of renal changes in diabetes. Such a classification may be useful both in clinical work and in research activities. Stage 1 ...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diab.32.2.s64
更新日期:1983-05-01 00:00:00
abstract::Canine pancreata obtained at total pancreatectomy were cannulated via the ducts and perfused with collagenase to prepare a tissue suspension that was isografted into the spleen (preparation congruent to 2 h, mean graft vol = 10 +/- 1 ml containing 24% of the B-cell mass/pancreas). In 13 dogs the tissue was implanted b...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.5.452
更新日期:1983-05-01 00:00:00
abstract::Counterregulatory hormonal responses were studied in six patients after 4-18 mo treatment with a continuous subcutaneous insulin infusion pump. In response to insulin-induced hypoglycemia, significant increases in epinephrine, norepinephrine, cortisol, and growth hormone were measured in all subjects, while in five of...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.5.398
更新日期:1983-05-01 00:00:00
abstract::Photoelectric plethysmography (PPG) was used to investigate blood flow changes close to superficial subcutaneous injection sites. As a validation procedure, the PPG response to subcutaneous injection of a known hyperemic agent, prostaglandin E1 (10(-5) M), was shown to correlate strongly with subcutaneous blood flow c...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.5.466
更新日期:1983-05-01 00:00:00
abstract::Cysteamine (300 mg/kg) administered subcutaneously depletes pancreatic somatostatin to 36% of control levels, but does not alter pancreatic insulin or glucagon content. Although perfusion of pancreata from normal animals with glucose (300 mg/dl) markedly stimulated somatostatin release, pancreata from cysteamine-treat...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.4.377
更新日期:1983-04-01 00:00:00
abstract::To explain the contradictory data on the secretion of prostacyclin (PGI2) in clinical and experimental diabetes, we have investigated the effect of each of the major metabolic abnormalities in uncontrolled diabetes on vascular PGI2 synthesis. An increase in fatty acid concentrations caused a dose-dependent inhibition ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.3.217
更新日期:1983-03-01 00:00:00
abstract::Peripheral somatic and autonomic nerve function have been studied in 79 teenage (16-19 yr) diabetics and 20 age- and sex-matched normal controls. Almost three-quarters of the diabetics (72%) had abnormal peripheral somatic nerve function tests, and one-third (31%) had abnormal cardiac parasympathetic tests. Both motor...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.2.142
更新日期:1983-02-01 00:00:00